Tuesday, June 2, 2020

Biology - Components of Eukaryotic cell

Components of Eukaryotic cell :

1. Cell wall: 

It is rigid, supportive and protective outer covering of plasma membrane of plant cells, fungi and some protists. Algae show presence of cellulose, galactans, mannans and minerals like calcium carbonate in cell wall. In other plants, it is made up of hemicelluloses, pectin, lipids and protein. Microfibrils of plant cell wall show presence of cellulose which is responsible for rigidity. Some of the depositions of cell wall are silica (grass stem), cutin (epidermal walls of land plants), suberin (endodermal cells of root), wax, lignin. It gives shape to the cell and protects from mechanical injury and infections.
Components of Eukaryotic cell
Plant cell

In plants, cell wall shows middle lamella, primary wall and secondary wall. Middle lamella : It is thin and lies between two adjacent cells. It is the first structure formed from cell plate during cytokinesis. It is mainly made up of pectin, calcium and magnesium pectate. Softening of ripe fruit is due to solubilization of pectin.
Components of Eukaryotic cell
Animal cell

Primary wall : 

In young plant cell, it is capable of growth. It is laid inside to middle lamella. It is the only wall seen in meristematic tissue, mesophyll, pith, etc.

Secondary wall : 

It is present inner to primary wall. Once the growth of primary wall stopps, secondary wall is laid. At some places thickening is absent which leads to formation of pits. Plasmodesmata are cytoplasmic bridges between neighbouring cells. It shows pores between cell wall and middle lamella.
Cell wall of plant cell

2. Cell membrane / Plasma membrane/  biomembrane : 

It is thin, quasifluid structure present both extracellularly and intracellularly. Extracellularly, it is present around protoplast and intracellularly, it is present around most of the cell organelles in eukaryotic cell. It separates cell organelles from cytosol. Thickness of biomembrane is about 75 A o. Under electron microscope, cell membrane appears trilaminar (made up of three layers). It shows presence of lipids (mostly phospholipids) arranged in bilayer. Lipids posses one hydrophilic polar head and two hydrophobic non-polar tails. So, phospholipids are amphipathic. Lipid molecules are arranged in two layers (bilayer) in such a way that their tails are sandwitched in between heads. Due to this, tails never come in direct contact with aqueous surrounding. Cell membrane also shows presence of proteins and carbohydrates. Ratio of proteins and lipids varies in different cells. For example - in human beings, RBCs show approximately 52% protein and 40% lipids. 
Components of Eukaryotic cell
Fluid mosaic model

Fluid mosaic model :

It is most accepted model of cell membrane. It was proposed by Singer and Nicholson in 1972. According to this model, it is made up of phospholipid bilayer and proteins. Proteins are like icebergs in the sea of lipids. Proteins can change their position. Some proteins are intrinsic i.e. occur at different depths of bilayer.

They span the entire thickness of the membrane. So, they are called transmembrane proteins. They form channels for passage of water. Extrinsic or peripheral proteins are found on two surfaces of the membrane. Quasifluid nature of lipid enables lateral movement of proteins. This ability to move within the membrane is measured as fluidity. Main function of plasma membrane is transport of molecules across it. This membrane is selectively permeable. During passive transport, many molecules move across the membrane without spending energy. Some solutes move by simple diffusion along the concentration gradient (from higher to lower concentration). Neutral solutes may move across the membrane by the process of simple diffusion This is called the passive transport. Water may also move by osmosis. During active transport, few ions or molecules are transported against concentration gradient (from lower to higher concentration). It requires energy. So, ATP is utilized. As such a transport is an energy dependent process in which ATP is utilized, it is called Active transport e.g. Na+/K+ pump. Polar molecules cannot pass through non-polar lipid bilayer. So, they require carrier proteins. 

3. Cytoplasm :

The cell contains ground substance called cytoplasmic matrix or cytosol. This colloidal jelly like material is not static. It shows streaming movements called cyclosis. The cytoplasm contains water as major component along with organic and inorganic molecules like sugars, amino acids, vitamins, enzymes, nucleotides, minerals and waste products. It also contains various cell organelles like endoplasmic reticulum, Golgi complex, mitochondria, plastids, nucleus, microbodies and cytoskeletal elements like microtubules. Cytoplasm acts as a source of raw materials as well as seat for various metabolic activities taking place in the cell. 

It helps in distribution and exchange of materials between various cell organelles. Cell organelles are nothing but compartments in the cell that carry out specific functions. Some of them coordinate with each other and complete specific tasks for the cell. Nuclear membrane, endoplasmic reticulum, Golgi complex, lysosomes and various types of vesicles and vacuoles form such a group and are together considered as endomembrane system of the cell. Organelles having distinct functions are not included in endomembrane system. e.g. mitochondria or chloroplast carry out specific type of energy conversions in the cell.

4. Endoplasmic Reticulum (ER):

This little network within the cytosol is present in all eukaryotic cells except ova and mature red blood corpuscles. Under the electron microscope, it appears like network of membranous tubules and sacs called cisternae. It forms more than 50% of the total membrane of a eukaryotic cell. This divides the cytoplasm in two parts viz; one within the lumen of ER called, laminal cytoplasm and non-laminal cytoplasm that lies outside ER. Membrane of Endoplasmic reticulum is continuous with nuclear envelope at one end and extends till cell membrane. It thus acts as intracellular supporting framework and helps in maintaining position of various cell organelles in the cytoplasm. The outer surface of endoplasmic reticular membrane may or may not be studded with ribosomes. Accordingly, it is called rough or smooth ER. Smooth and rough ER differ in their functions. Smooth ER is involved in various processes in different cells. Depending on cell type, it helps in synthesis of lipids (ex. steroid secreting cells of cortical region of adrenal gland, testes and ovaries), detoxification of drugs and poisons (liver cells) and storage of calcium ions (muscle cells).
Components of Eukaryotic cell
Endoplasmic reticulum

Rough ER is primarily involved in protein synthesis. (e.g. pancreatic cells that secrete insulin). These proteins are secreted by ribosomes attached to rough ER and are called secretory proteins. These proteins get wrapped in membrane that buds off from transitional region of ER. Such membrane bound proteins depart from ER as transport vesicles. Rough ER is also involved in formation of membrane for the cell. The ER membrane grows in place by addition of membrane proteins and phospholipids to its own membrane. Portions of this expanded membrane are transferred to other components of endomembrane system.

5. Golgi complex : 

Golgi complex or Golgi apparatus or Golgi body; various terms are used to denote this assembly, manufacturing cum packaging and transport unit of cell. Golgi complex essentially consists of stacks of membranous sacs called cisternae. Diameter of cisternae varies from 0.5 to 1 ┬Ám. A cell may have few to several cisternae depending on its function. The thickness and molecular composition of two membranes of a Golgi sac differ from each other. The Golgi sacs show specific orientation in the cell. Each cisterna has a forming or ‘cis’ face (cis: on the same side) and maturing or ‘ trans’ face (trans: the opposite side). Transport vesicles that pinch off from transitional ER merge with cis face of Golgi cisterna and add its contents into the lumen.
Components of Eukaryotic cell
Golgi complex

This explains why Golgi bodies are usually located near ER. Modified and condensed secretions leave Golgi through trans face again as membrane bound vesicles. Golgi body carries out two types of functions, modification of secretions of ER and production of its own secretions. Cisternae contain specific enzymes for specific functions. Refining of product takes place in an orderly manner. For example, glycolipids and glycoproteins that are brought from ER loose certain sugars and regain other, thus forming a variety of products. Golgi bodies also manufacture their own products. Golgi bodies in many plant cells produce non-cellulose polysaccharides like pectin. Manufactured or modified, all products of Golgi complex leave cisternae from trans face as transport vesicles. 

While they are leaving from the Golgi, certain markers may get impregnated on their membrane so that they can identify their specific target cell or cell organelle.

6. Lysosomes : 

Lysosomes can be considered as dismantling and restructuring units of a cell. These are membrane bound vesicles containing hydrolytic enzymes. The enzymes in lysosomes are used by most eukaryotic cells to digest (hydrolyse) macromolecules. The lysosomal enzymes show optimal activity in acidic pH. Lysosomes arise from Golgi associated endoplasmic reticulum. The list of lysosomal enzymes includes all types of hydrolases viz, amylases, proteases and lipases. These enzymes are in inactive state and are activated only when a lysosome comes in contact with another particular organelle to form a hybrid structure. After the action of enzymes is over, the lysosome is reformed and re-used. Lysosomes are thus found in various structural forms and carry out various functions for the cell. 

Lysosomes are polymorphic in nature. We can classify lysosomes as, Primary lysosomes; which are nothing but membrane bound vesicles in which enzymes are in inactive state.
Secondary lysosomes or hybrid lysosomes, which are formed by fusion of lysosome with endocytic vesicle containing materials to be digested, represented as heterophagic vesicle. This is larger in size than primary lysosome. Residual body is the vesicle containing undigested remains left over in the heterophagic vesicle after releasing the products of digestion in the cytosol. Lysosomes which bring about digestion of cells own organic material like a damaged cell organelle, are called autophagic vesicles (or suicide bags). An autophagic vesicle essentially consists of lysosome fused with membrane bound old cell organelle or organic molecules to be recycled. Remember, every week, a human liver cell recycles half of its macromolecules. Lysosomes bring about intracellular and extracellular digestion. The intracellular digestion is brought about by autophagic vesicle or secondary lysosomes which contain foreign materials brought in by processes like phagocytosis. e.g. Food vacuole in amoeba or macrophages in human blood that engulf and destroy harmful microbes that enter the body.

Extracellular digestion is brought about by release of lysosomal enzymes outside the cell. e.g. acrosome, a cap like structure in human sperm is a modified lysosome which contain various enzymes like Hyaluronidase. These enzymes bring about fertilization by dissolving protective layers of ovum. During metamorphosis process found in many organisms, lysosomal enzymes help in reusing the tissues of redundant organs. They also help in destruction of malignant cells. e.g. T-lymphocytes.

7. Vacuoles : 

Vacuoles are membrane bound sacs prominently found in plant cells. In animal cells, whenever present they are few in number and smaller in size. Generally, there are two or three permanent vacuoles in a plant cell. In some large plant cells, a single large vacuole occupies the central part of the cell. It is called central vacuole. In such cells vacuole can occupy as much as 90% of the total volume of the cell. The vacuoles are bound by semipermeable membrane, called tonoplast membrane. This membrane helps in maintaining the composition of vacuolar fluid; the cell sap, different from that of the cytosol. Composition of cell sap differs in different types of cells. The cell sap of central vacuole is a store house of various ions and thus is hypertonic to cytosol. Small vacuoles in seeds of certain plants store organic materials like proteins. Vacuoles store excretory products or even compounds that are harmful or unpalatable to herbivores, thereby protecting the plants. Attractive colours of the petals are due to storage of such pigments in vacuoles. Intake of food or foreign particle by phagocytosis involves formation of food vacuole. In fresh water unicellular forms like Paramoecium, excretion and osmoregulation takes place by contractile vacuoles. Vacuoles maintain turgidity of the cell.

8. Glyoxysomes : 

These membrane bound organelles contain enzymes that convert fatty acids to sugar. They can be observed in cells of germinating seeds where the cells utilise stored fats as source of sugar till it starts photosynthesising on its own.
Components of Eukaryotic cell

9. Mitochondria (Singular :  Mitochondrion) :

These are important cell organelles involved in aerobic respiration. Mitochondria are absent in prokaryotic cells and red blood corpuscles (RBCs). Their shape may be oval or spherical or spiral strip like. It is a double membrane bound organelle. Outer membrane is permeable to various metabolites due to presence of a protein -Porin or Parson’s particles. Inner membrane is selectively permeable to few substances only. Both membranes are separated by a space outer chamber. Inner membrane shows several finger like or plate like folds- cristae. Inner membrane bears numerous particles- oxysomes and cytochromes / electron carriers. Inner membrane encloses a cavity- inner chamber, containing a fluid- matrix. Matrix contains few coils of circular DNA, RNA, 70S types of ribosomes, lipids and various enzymes of Krebs cycle and other pathways.

Oxysomes : 

Inner membrane of mitochondria bears numerous particles - Oxysomes (F1-F0 / Fernandez - Moran / Elementary particles / mitochondrial particles). Each particle consists 
of head and stalk / foot. Head (F1) / lollipop head faces towards matrix and foot (F0) is embedded in inner membrane. Head acts as an enzyme ATP synthase and foot as proton channel. Oxysomes are involved in proton pumping and ATP synthesis.

10. Plastids : 

Like mitochondria, plastids too are double walled organelles containing DNA, RNA and 70S ribosomes. But they are larger in size and can be observed under light microscope. Plastids are classified according to the pigments present in it as leucoplasts, chromoplasts and chloroplasts. Leucoplasts do not contain any pigments, they are of various shapes and sizes. These are meant for storage of nutrients. e.g. Amyloplasts that store starch, Elaioplasts that store oils and Aleuroplasts that store proteins.

Chromoplasts contain pigments like carotene and xanthophyll, etc. They impart red, yellow or orange colour to flowers and fruits. Now you must have understood why potato looks white in colour and shoe flower petals are red. Both leucoplasts and amyloplasts donot contain photosynthetic apparatus we find in chloroplasts. Let us now study the chloroplast in details. Plant cells, cells of algae and few protists like Euglena contain chloroplasts. You have observed ribbon shaped chloroplast in Spirogyra. It differs in size, number and shape in various cells in which it is found. In plants, it is found in green regions; mainly in mesophyll 
of leaf. This chloroplast is lens shaped. But it can also be oval, spherical, discoid or ribbon like. A cell may contain single large chloroplast as in Chlamydomonas or there are 20 to 40 chloroplasts per cell seen in mesophyll cells. Chloroplasts contain green pigment - chlorophyll along with other enzymes that help in production of sugar by photosynthesis.Inner membrane of double walled chlorophyll is comparatively less permeable. Inside the cavity of inner membrane, there is another set of membranous sacs called thylakoids. Thylakoids are arranged in the form of stacks called grana (singular: granum). The grana are connected to each other by means of membranous tubules called stroma lamellae. Space outside thylakoids is is filled with stroma. The stroma, and the space inside thylakoids contain various enzymes essential for photosynthesis. Like other plastids, stroma of chloroplast also contains DNA and ribosomes.

11. Ribosomes :

You are aware that ribosomes are protein factories of the cell. They use the genetic information to synthesise proteins. Ribosomes were first observed as dense particles in electron micrograph of a cell by scientist Pallade in 1953. Ribosomes are made up of Ribosomal RNA and proteins. They do not have any membranous covering around them. 
Components of Eukaryotic cell

In a eukaryotic cell, ribosomes are present in mitochondria, plastids and in cytosol. Ribosomes in cytoplasm are either found attached to outer surface of Rough Endoplasmic Reticulum and nuclear membrane or freely suspended in cytoplasm. Both are similar in structure and are 80S type. Each ribosome is made up of two subunits; a large and a small subunit. Bound ribosomes generally produce proteins that are transported outside the cell after processing in ER and Golgi body. e.g. Bound ribosomes of acinar cells of pancreas produce pancreatic digestive enzymes. Free ribosome come together and form chains called polyribosomes for protein synthesis.

Free ribosomes generally produce enzymatic proteins that are used up in cytoplasm like enzymes required for breakdown of sugar. Both types of ribosomes can interchange position and function. Number of ribosomes is high in cells actively engaged in protein synthesis. 

12. Nucleus :

Structure of nucleus of a eukaryotic cell becomes distinct in a non-dividing cell or during interphase. Such an interphase nucleus is made up of nuclear envelope, nucleoplasm, nucleolus and chromatin network. Nuclear envelope is a double walled delimiting membrane of nucleus. Two membranes are separated from each other by perinuclear space (10 to 50nm). Outer membrane is connected with endoplasmic reticulum at places. It also harbours ribosomes on it. The inner membrane is lined by nuclear lamina- a network of protein fibres that helps in maintaining shape of the nucleus. The two membranes along with perinuclear space help in separating nucleoplasm from cytoplasm. However, nuclear membrane is not continuous. At places, there are small openings called nucleopores. The nucleopores are guarded by pore complexes which regulate flow of substances from nucleus to cytoplasm and in reverse direction. 

The nucleoplasm or karyolymphcontains various substances like nucleic acids, protein molecules, minerals and salts. It contains chromatin network and nucleolus. Nucleolus is another component which is not bound by cell membrane. Nucleolus is made up of rRNA and ribosomal proteins and it is best known as the site of ribosome biogenesis. Depending on synthetic activity of a cell, there are one or more nucleoli present in the nucleoplasm. For ex: cells of oocyte contain large nucleolus whereas sperm cells contain small inconspicuous one. They appear as dense spherical bodies present near chromatin network. They produce rRNA and ribosomal proteins which are then transported to cytoplasm and are assembled together to form ribosomes. 

Chromatin material : 
in the form of chromosomes which are nothing but DNA molecules associated with proteins. In a nondividing cell, the chromosomes appear as thread like network and cannot be identified individually. This network is called chromatin material. The chromatin material contains DNA, histone and non-histone proteins and RNA. In some regions of chromatin, DNA is more and is genetically active called euchromatin. Some regions that contain more of proteins and less DNA and are genetically inert, are called heterochromatin.
Components of Eukaryotic cell
Ultrastructure of nucleus

When the cell prepares to divide, the chromosomes coil and get condensed. At metaphase stage, they become distinct and can be clearly identified. You will study this process as well as structure and types of chromosome in other chapters. Every species of living organism has specific number of chromosomes like normal human cell has 46. The nucleus contains entire genetic information, hence play important role in heredity and variation. It is the site for synthesis of DNA, RNA and ribosomes. It plays important role in protein synthesis. Chromosome number being constant for a species, it is important in phylogenetic studies. Nucleus thus is the master cell organelle. 

13. Cytoskeleton : 

With advancement in light and electron microscopy, scientists revealed presence of network of fibrils throughout the cytoplasm. It is called cytoskeleton. Cytoskeleton consists of microtubules, microfilaments and intermediate filaments. Microtubules are made up of protein- tubulin. Microfilaments are made up of actin and intermediate filaments are composed of fibrous proteins. Cytoskeleton helps in maintenance of shape of cell, contraction of cell, mobility of cell and cell organelles, changes in shape of the cells and cell division.

Cilia and flagella :

They are fine hair like membrane bound protoplasmic outgrowths that occur on the free surface of the cell. They generate a current in fluid medium for passage of material and locomotion. Cilia are small in size and many in number. Cilia act as oars causing movement of cell. Flagella are longer and few in number. Flagella present in prokaryotic bacteria are structurally different from that of eukaryotic flagella. Cilium or flagellum consists of basal body, basal plate and shaft. Basal body is placed in outer part of cytoplasm. It is derived from centriole. It has nine peripheral triplets of fibrils. Shaft is exposed part of cilia or flagella. It consists of two parts- sheath and axoneme. Sheath is covering membrane of cilium or flagellum.
Components of Eukaryotic cell
Structure of Cilia

Core called axoneme possesses 11 fibrils running parallel to long axis. It shows 9 peripheral doublets and two single central fibrils (9+2). The central tubules are enclosed by central sheath. This sheath is connected to one of the tubules of peripheral doublets by a radial spoke. Central tubules are connected to each other by bridges. The peripheral doublets are connected to each other through linkers or interdoublet bridge.

Centrioles and centrosomes :
Centrosome is usually found near the nucleus of an animal cell. It contains a pair of cylindrical structures called centrioles. The cylinders are perpendicular to each other and are surrounded by amorphous substance called pericentriolar material. Each cylinder of centriole is made up of nine sets of triplet microtubules made up of tubulin. Evenly spaced triplets are connected to each other by means of non-tubulin proteins. 
Structure of Centriole

At the proximal end of centriole, there is a set of tubules called hub. The peripheral triplets are connected to hub by means of radial spokes. Due to this proximal end of centriole looks like a cartwheel. The centrosomes help in assembly of spindle apparatus during cell division. It forms basal body of cilia and flagella.


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